Application

Western blot: 1:2,000

ELISA

Optimal working dilutions must be determined by end user.

Research Sub CategoryEpitope Tags

Detect Polyhistidine Tag using this Anti-Polyhistidine Tag Antibody validated for use in ELISA & WB.

Research CategoryEpitope Tags & General Use

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

His tagged recombinant protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB3112

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin. Liquid containing 0.1% sodium azide.

Specificity

Reacts with the epitope MRGSHHHHHH which is present in pQE vectors. It is expected that the antibody will also react with proteins expressed from vectors such as PRSET A, B, and C. The antibody should not react with pAC, N, T, A, B, C baculovirus vectors.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.